Article
Converting Escherichia coli RNA polymerase into an enhancer-responsive enzyme: role of an NH2-terminal leucine patch in sigma 54.
Science (New York, N.Y.) - 10 Nov 1995
Wang J T, Syed A, Hsieh M, Gralla J D
Abstract excerpt
The protein sigma 54 associates with Escherichia coli core RNA polymerase to form a holoenzyme that binds promoters but is inactive in the absence of enhancer activation. Here, mutants of sigma 54 enabled polymerases to transcribe without enhancer protein and adenosine triphosphate. The mutations...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Bacterial Proteins
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- Enhancer Elements, Genetic
- Escherichia coli
- Escherichia coli Proteins
- Leucine
- Molecular Sequence Data
- Mutation
- PII Nitrogen Regulatory Proteins
- Promoter Regions, Genetic
- RNA Polymerase Sigma 54
- Sigma Factor
- Trans-Activators
- Transcription Factors
- Transcription, Genetic