Mutant forms of Salmonella typhimurium sigma54 defective in transcription initiation but not promoter binding activity.

Abstract excerpt

Transcription initiation with sigma54-RNA polymerase holoenzyme (sigma54-holoenzyme) has absolute requirements for an activator protein and ATP hydrolysis. sigma54's binding to core RNA polymerase and promoter DNA has been well studied, but little is known about its role in the subsequent steps of transcription initiation. Following random mutagenesis, we isolated eight mutant forms of Salmonella typhimurium...

Topics

• Alleles
• Amino Acid Substitution
• Bacterial Proteins
• Bacteriophage P22
• DNA Mutational Analysis
• DNA Probes
• DNA-Binding Proteins
• DNA-Directed RNA Polymerases
• Gene Expression Regulation, Bacterial
• Genes, Reporter
• Glutamate-Ammonia Ligase
• Glutamine
• Holoenzymes
• Mutation