Article
Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11.
Protein engineering - 1 Mar 1995
delCardayré S B, Ribó M, Yokel E M, Quirk D J, Rutter W J, Raines R T
Abstract excerpt
Bovine pancreatic ribonuclease A (RNase A) has been the object of much landmark work in biological chemistry. Yet the application of the techniques of protein engineering to RNase A has been limited by problems inherent in the isolation and heterologous expression of its gene. A cDNA library was...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- Cattle
- DNA, Complementary
- Escherichia coli
- Gene Library
- Genetic Vectors
- Glutamine
- Glycosylation
- Kinetics
- Molecular Sequence Data
- Mutation
- Pancreas
- Plasmids
- Protein Engineering
- Protein Processing, Post-Translational