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Article

Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11.

Protein engineering - 1 Mar 1995

delCardayré S B, Ribó M, Yokel E M, Quirk D J, Rutter W J, Raines R T

Abstract excerpt

Bovine pancreatic ribonuclease A (RNase A) has been the object of much landmark work in biological chemistry. Yet the application of the techniques of protein engineering to RNase A has been limited by problems inherent in the isolation and heterologous expression of its gene. A cDNA library was prepared from cow pancreas, and from this library the cDNA that codes for RNase A was isolated. This cDNA was inserted...

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PMID: 7479688
DOI: 10.1093/protein/8.3.261

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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