Expression of wild-type and mutant bovine pancreatic ribonuclease A in Escherichia coli.

doi:10.1073/pnas.90.2.615

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Expression of wild-type and mutant bovine pancreatic ribonuclease A in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America - 15 Jan 1993

Laity J H, Shimotakahara S, Scheraga H A

Abstract excerpt

Wild-type ribonuclease A and five mutants thereof have been expressed in Escherichia coli as fusion proteins by using a T7 expression system. The five mutants are C[65-72]S, C[40-95]S, C[58-110]S, C[26-84]S, and K41G. The expressed fusion protein formed inclusion bodies which were then cleaved by...

Topics

Amino Acid Sequence
Animals
Bacteriophage T7
Base Sequence
Cattle
Circular Dichroism
Cytoplasmic Granules
Escherichia coli
Factor Xa
Genetic Vectors
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Promoter Regions, Genetic

Protein Folding

Protein Precursors

Protein Processing, Post-Translational

Recombinant Fusion Proteins

Ribonuclease, Pancreatic

Source

Open in PubMed Open full text

Identifiers

PMID: 8421696
DOI: 10.1073/pnas.90.2.615
PMCID: PMC45714

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8421696-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Expression of wild-type and mutant bovine pancreatic ribonuclease A in Escherichia coli.PMID 8421696Read ↗

Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11.Semantic proximity Read ↗

Significant stabilization of ribonuclease A by additive effects.Semantic proximity Read ↗

Crystal structures of two mutants that have implications for the folding of bovine pancreatic ribonuclease A.Semantic proximity Read ↗

In vitro evolution of a dimeric variant of human pancreatic ribonuclease.Semantic proximity Read ↗

Stabilization of human pancreatic ribonuclease through mutation at its N-terminal edge.Semantic proximity Read ↗

Folding and unfolding kinetics of the proline-to-alanine mutants of bovine pancreatic ribonuclease A.Semantic proximity Read ↗

Regeneration of three-disulfide mutants of bovine pancreatic ribonuclease A missing the 65-72 disulfide bond: characterization of a minor folding pathway of ribonuclease A and kinetic roles of Cys65 and Cys72.Semantic proximity Read ↗

A thermoresistant mutant of ribonuclease T1 having three disulfide bonds.Semantic proximity Read ↗

Increased proteolytic resistance of ribonuclease A by protein engineering.Semantic proximity Read ↗

Effects of tyrosine mutations on the conformational and oxidative folding of ribonuclease a: a comparative study.Semantic proximity Read ↗

Substitution of a conserved disulfide in the type IIa bacteriocin, leucocin A, with L-leucine and L-serine residues: effects on activity and three-dimensional structure.Semantic proximity Read ↗

Analysis by systematic deletion of amino acids of the action of the ribotoxin restrictocin.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.