Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Expression of wild-type and mutant bovine pancreatic ribonuclease A in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America - 15 Jan 1993

Laity J H, Shimotakahara S, Scheraga H A

Abstract excerpt

Wild-type ribonuclease A and five mutants thereof have been expressed in Escherichia coli as fusion proteins by using a T7 expression system. The five mutants are C[65-72]S, C[40-95]S, C[58-110]S, C[26-84]S, and K41G. The expressed fusion protein formed inclusion bodies which were then cleaved by factor Xa. The cleaved ribonuclease A was isolated as unfolded (sulfonated), soluble protein which was subsequently...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to Base Sequence

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Cattle

Share to Escherichia coli

Share to Molecular Sequence Data

Share to Mutation

Share to Promoter Regions, Genetic

Share to Recombinant Fusion Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 8421696
DOI: 10.1073/pnas.90.2.615
PMCID: PMC45714

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Expression of wild-type and mutant bovine pancreatic ribonuclease A in Escherichia coli.PMID 8421696Read ↗

Engineering ribonuclease A: production, purification and characterization of wild-type enzyme and mutants at Gln11.Semantic proximity Read ↗

Significant stabilization of ribonuclease A by additive effects.Semantic proximity Read ↗

Crystal structures of two mutants that have implications for the folding of bovine pancreatic ribonuclease A.Semantic proximity Read ↗

In vitro evolution of a dimeric variant of human pancreatic ribonuclease.Semantic proximity Read ↗

Stabilization of human pancreatic ribonuclease through mutation at its N-terminal edge.Semantic proximity Read ↗

Folding and unfolding kinetics of the proline-to-alanine mutants of bovine pancreatic ribonuclease A.Semantic proximity Read ↗

Regeneration of three-disulfide mutants of bovine pancreatic ribonuclease A missing the 65-72 disulfide bond: characterization of a minor folding pathway of ribonuclease A and kinetic roles of Cys65 and Cys72.Semantic proximity Read ↗

A thermoresistant mutant of ribonuclease T1 having three disulfide bonds.Semantic proximity Read ↗

Increased proteolytic resistance of ribonuclease A by protein engineering.Semantic proximity Read ↗

Effects of tyrosine mutations on the conformational and oxidative folding of ribonuclease a: a comparative study.Semantic proximity Read ↗

Substitution of a conserved disulfide in the type IIa bacteriocin, leucocin A, with L-leucine and L-serine residues: effects on activity and three-dimensional structure.Semantic proximity Read ↗

Analysis by systematic deletion of amino acids of the action of the ribotoxin restrictocin.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.