Article
Protein engineering of ribonucleases.
Biochimie - 1 Nov 1998
Cafaro V, Bracale A, Formiggini F, Notomista E, D'Alessio G, Di Donato A
Abstract excerpt
Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric RNases display selective toxicity for malignant cells, which is absent in the monomeric mutants.
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.