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Article

A DNA binding motif coordinating synthesis and degradation in proofreading DNA polymerases.

The EMBO journal - 1 Jul 1996

Truniger V, Lázaro J M, Salas M, Blanco L

Abstract excerpt

The functional significance of the conserved motif 'YxGG/A', located between the 3'-5' exonuclease and polymerization domains of eukaryotic-type DNA polymerases, has been studied by site-directed mutagenesis in phi29 DNA polymerase. Single substitutions at this region were obtained, and 11 phi29 DNA polymerase mutant derivatives were overproduced in Escherichia coli and purified to homogeneity. Nine mutants...

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Identifiers

PMID: 8670845
PMCID: PMC451907

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A DNA binding motif coordinating synthesis and degradation in proofreading DNA polymerases.PMID 8670845Read ↗

Involvement of phi29 DNA polymerase thumb subdomain in the proper coordination of synthesis and degradation during DNA replication.Semantic proximity Read ↗

Noncatalytic aspartate at the exonuclease domain of proofreading DNA polymerases regulates both degradative and synthetic activities.Semantic proximity Read ↗

ø29 DNA polymerase residue Lys383, invariant at motif B of DNA-dependent polymerases, is involved in dNTP binding.Semantic proximity Read ↗

DNA stabilization at the Bacillus subtilis PolX core--a binding model to coordinate polymerase, AP-endonuclease and 3'-5' exonuclease activities.Semantic proximity Read ↗

phi29 DNA polymerase residue Phe128 of the highly conserved (S/T)Lx(2)h motif is required for a stable and functional interaction with the terminal protein.Semantic proximity Read ↗

Phage phi 29 DNA polymerase residues involved in the proper stabilisation of the primer-terminus at the 3'-5' exonuclease active site.Semantic proximity Read ↗

phi 29 DNA polymerase residue Leu384, highly conserved in motif B of eukaryotic type DNA replicases, is involved in nucleotide insertion fidelity.Semantic proximity Read ↗

An invariant lysine residue is involved in catalysis at the 3'-5' exonuclease active site of eukaryotic-type DNA polymerases.Semantic proximity Read ↗

Involvement of the "linker" region between the exonuclease and polymerization domains of phi29 DNA polymerase in DNA and TP binding.Semantic proximity Read ↗

A positively charged residue of phi29 DNA polymerase, highly conserved in DNA polymerases from families A and B, is involved in binding the incoming nucleotide.Semantic proximity Read ↗

The highly conserved amino acid sequence motif Tyr-Gly-Asp-Thr-Asp-Ser in alpha-like DNA polymerases is required by phage phi 29 DNA polymerase for protein-primed initiation and polymerization.Semantic proximity Read ↗

Fidelity of DNA replication-a matter of proofreading.Semantic proximity Read ↗

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