Article
A DNA binding motif coordinating synthesis and degradation in proofreading DNA polymerases.
The EMBO journal - 1 Jul 1996
Truniger V, Lázaro J M, Salas M, Blanco L
Abstract excerpt
The functional significance of the conserved motif 'YxGG/A', located between the 3'-5' exonuclease and polymerization domains of eukaryotic-type DNA polymerases, has been studied by site-directed mutagenesis in phi29 DNA polymerase. Single substitutions at this region were obtained, and 11 phi29...
Topics
- Amino Acid Sequence
- Bacillus Phages
- Base Sequence
- Binding Sites
- DNA Primers
- DNA, Single-Stranded
- DNA, Viral
- DNA-Directed DNA Polymerase
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Templates, Genetic