Article
Systems for generating and detecting mutations in the galactose operon promoter region.
Annales de microbiologie - 1 Jan 2000
Busby S, Irani M, Adhya S, de Crombrugghe B
Abstract excerpt
We discuss the use of plasmid systems which enable us to generate and detect mutations in the Escherichia coli galactose operon promoter region. We describe the isolation of mutants which map the galactose promoters and which pinpoint the sites for the binding of the cyclic AMP-binding protein (CRP) and the galactose repressor. Finally, we describe how such mutations can be transferred from the plasmid systems to...
Topics
- Bacterial Proteins
- Base Sequence
- Carrier Proteins
- Cloning, Molecular
- Cyclic AMP Receptor Protein
- DNA, Recombinant
- DNA-Directed RNA Polymerases
- Escherichia coli
- Galactose
- Mutation
- Operon