Segment-specific mutagenesis of the regulatory region in the Escherichia coli galactose operon: isolation of mutations reducing the initiation of transcription and translation.

Abstract excerpt

Using hydroxylamine mutagenesis in vitro, mutations were introduced into a short DNA fragment containing the two overlapping promoters of the Escherichia coli galactose operon and the start of the first gal gene, galE. The mutagenised fragment was inserted into a lac expression plasmid. In such a vector, lac expression is controlled by the gal promoter region. Amongst eighteen candidates in which expression was...

Topics

• Base Sequence
• DNA Restriction Enzymes
• Escherichia coli
• Galactose
• Hydroxylamine
• Hydroxylamines
• Mutagens
• Mutation
• Operon
• Plasmids
• Protein Biosynthesis