Article
A novel RNA polymerase binding site upstream of the galactose promoter in Escherichia coli exhibits promoter-like activity.
European journal of biochemistry - 1 Apr 2001
Sur R, Debnath D, Mukhopadhyay J, Parrack P
Abstract excerpt
RNA polymerase is known to bind and utilize the overlapping promoters P1 and P2 in Escherichia coli galactose operon. We have identified an additional specific site upstream of P2, where RNA polymerase binds in a heparin-resistant manner. Binding of polymerase to this site, termed P3, occurs simultaneous to its binding at P1/P2. We have located this P3 site by DNase I footprinting. A 63 base pair region centered...
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