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Article

Galactose utilization in Lactobacillus helveticus: isolation and characterization of the galactokinase (galK) and galactose-1-phosphate uridyl transferase (galT) genes.

Journal of bacteriology - 1 Jul 1991

Mollet B, Pilloud N

Abstract excerpt

By complementing appropriate gal lesions in Escherichia coli K802, we were able to isolate the galactokinase (galK) and galactose-1-phosphate uridyl transferase (galT) genes of Lactobacillus helveticus. Tn10 transposon mutagenesis, together with in vivo complementation analysis and in vitro enzyme activity measurements, allowed us to map these two genes. The DNA sequences of the genes and the flanking regions...

Topics

Amino Acid Sequence
Base Sequence
Escherichia coli
Galactokinase
Galactose
Genes, Bacterial
Genotype
Lactobacillus
Molecular Sequence Data
Mutagenesis, Insertional
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Biosynthesis

Restriction Mapping

Sequence Homology, Nucleic Acid

Transcription, Genetic

UTP-Hexose-1-Phosphate Uridylyltransferase

Source

Open in PubMed Open full text

Identifiers

PMID: 2066342
DOI: 10.1128/jb.173.14.4464-4473.1991
PMCID: PMC208110

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2066342-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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