Specific sequences downstream from -6 are not essential for proper and efficient in vitro utilization of the Escherichia coli lactose promoter.

doi:10.1016/s0022-2836(05)80319-5

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Article

Specific sequences downstream from -6 are not essential for proper and efficient in vitro utilization of the Escherichia coli lactose promoter.

Journal of molecular biology - 20 Nov 1990

Lorimer D D, Cao J L, Revzin A

Abstract excerpt

A series of deletion mutants of the wild-type Escherichia coli lactose promoter, with endpoints at +25, +19, +14, +1 and -6 (relative to the start of transcription at +1), was constructed and the deleted DNA replaced with non-lac DNA. These mutants were used to show that no specific DNA sequences...

Topics

Base Sequence
Chromosome Deletion
DNA, Bacterial
DNA-Directed RNA Polymerases
Escherichia coli
Genes, Bacterial
Kinetics
Lactose
Molecular Sequence Data
Mutation
Promoter Regions, Genetic
Restriction Mapping
Transcription, Genetic

Source

Open in PubMed

Identifiers

PMID: 2254929
DOI: 10.1016/s0022-2836(05)80319-5

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2254929-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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