Article
Pyrimidine dimer-DNA glycosylases: studies on bacteriophage T4-infected and on uninfected Escherichia coli.
Biochimie - 1 Jan 2000
Bonura T, Radany E H, McMillan S, Love J D, Schultz R A, Edenberg H J, Friedberg E C
Abstract excerpt
Pyrimidine dimer (PD)-DNA glycosylase activity has been reported in both the M. luteus and phage T4 UV endonucleases. In the present studies the T4 PD-DNA glycosylase has been purified close to physical homogeneity using an assay that measures the release of free thymine from UV-irradiated poly ([H5] dT):poly (dA), after the photo-reversal of thymine-thymine dimers. The activity has also been demonstrated in vivo...
Topics
- DNA Glycosylases
- DNA Polymerase I
- DNA-(Apurinic or Apyrimidinic Site) Lyase
- Deoxyribonuclease IV (Phage T4-Induced)
- Endodeoxyribonucleases
- Escherichia coli
- Escherichia coli Proteins
- Kinetics
- Mutation
- N-Glycosyl Hydrolases