Article
Roles of uracil-DNA glycosylase and apyrimidinic endonucleases in the molecular 5-bromo-2'-deoxyuridine photosensitization in Escherichia coli K-12.
Photochemistry and photobiology - 1 Jul 1993
Yamamoto Y, Fujiwara Y
Abstract excerpt
The molecular mechanism for 5-bromo-2'-deoxyuridine (BrdU) photosensitization was studied in thymine-requiring wild-type and uracil-DNA glycosylase (UDG)-deficient ung mutant cells of Escherichia coli K-12. Wild-type cells were more sensitive to BrdU photosensitization than ung mutant cells. UV induced the identical numbers of alkaline sucrose single-strand breaks (SSB) in 5-bromouracil-DNA (BrU-DNA) of both the...
Topics
- Bromodeoxyuridine
- DNA Glycosylases
- DNA, Bacterial
- DNA-(Apurinic or Apyrimidinic Site) Lyase
- Deoxyribonuclease IV (Phage T4-Induced)
- Endodeoxyribonucleases
- Escherichia coli
- Escherichia coli Proteins
- Mutation
- N-Glycosyl Hydrolases