Initiation of DNA synthesis in the transfer origin region of RK2 by the plasmid-encoded primase: detection using defective M13 phage.

doi:10.1016/0147-619x(90)90048-h

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Article

Initiation of DNA synthesis in the transfer origin region of RK2 by the plasmid-encoded primase: detection using defective M13 phage.

Plasmid - 1 Jan 1990

Yakobson E, Deiss C, Hirata K, Guiney D G

Abstract excerpt

The broad host range IncP (IncP1) plasmids of gram-negative bacteria encode DNA primases that are involved in conjugal DNA synthesis. The primase of RK2/RP4 is required for efficient DNA transfer to certain gram-negative bacteria, indicating that the enzyme primes complementary strand synthesis i...

Topics

Bacteriophages
Conjugation, Genetic
DNA Primase
DNA Replication
DNA, Bacterial
Mutation
Plasmids
RNA Nucleotidyltransferases
Restriction Mapping
Transfection

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