Article
Uracil-DNA glycosylase causes 5-bromodeoxyuridine photosensitization in Escherichia coli K-12.
Journal of bacteriology - 1 Sept 1990
Yamamoto Y, Fujiwara Y
Abstract excerpt
An Escherichia coli uracil-DNA glycosylase-defective mutant (ung-1 thyA) was more resistant than its wild-type counterpart (ung+ thyA) to the killing effect of UV light when cultured in medium containing 5-bromouracil or 5-bromo-2'-deoxyuridine (BrdUrd). The phenotype of resistance to BrdUrd photosensitization and the uracil-DNA glycosylase deficiency appeared to be 100% cotransduced by P1 phage. During growth...
Topics
- Bromodeoxyuridine
- Chromatography, High Pressure Liquid
- DNA Glycosylases
- DNA, Bacterial
- Dose-Response Relationship, Radiation
- Escherichia coli
- Mutation
- N-Glycosyl Hydrolases
- Phenotype
- Ultraviolet Rays
- Uracil-DNA Glycosidase
