Article
A chromosomal expression vector for Escherichia coli based on the bacteriophage Mu.
Gene - 15 Apr 1993
Weinberg R A, De Ciechi P A, Obukowicz M
Abstract excerpt
A new Escherichia coli expression vector with increased stability was developed based on bacteriophage Mu. Unlike traditional expression vectors, the vector described herein is chromosome based rather than existing as an autonomously replicating plasmid. The chromosomal location resulted in extreme stability of the vector even in the absence of selective pressure. Both replication and heterologous protein...
Topics
- Animals
- Bacteriophage mu
- Base Sequence
- Blotting, Southern
- Chromosomes, Bacterial
- Cloning, Molecular
- Culture Media
- DNA, Single-Stranded
- Escherichia coli
- Gene Expression Regulation
- Genetic Variation