Use of a novel cassette to label phenotypically a cryptic plasmid of Bacillus subtilis and map loci involved in its stable maintenance.

doi:10.1099/00221287-136-5-905

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Article

Use of a novel cassette to label phenotypically a cryptic plasmid of Bacillus subtilis and map loci involved in its stable maintenance.

Journal of general microbiology - 1 May 1990

Gleave A P, Mountain A, Thomas C M

Abstract excerpt

In order to facilitate studies on the maintenance of cryptic plasmids from Gram-positive bacteria we have constructed a novel cassette cAPG1000 (5.0 kb) which carries both a selectable marker (chloramphenicol resistance from Staphylococcus aureus plasmid pC194) and a screenable marker (the xylE g...

Topics

Bacillus subtilis
Chromosome Mapping
Genetic Markers
Hybridization, Genetic
Mutation
Phenotype
Plasmids

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