Article
Chromosome rearrangements induced by recombinant coliphage lambda placMu.
Gene - 28 Sept 1990
Barr G C, Dorman C J, Mellor J, Higgins C F
Abstract excerpt
Operon fusions to lacZ, commonly used to study bacterial gene expression in vivo, are normally constructed using phage derivatives such as lambda placMu53 or Mud-1. These derivatives contain a part of trp operon, and we have found that, when integrated into the chromosome, recombination can occur at high frequency between this trp DNA and the chromosomal trp operon leading to chromosomal inversions which fuse...
Topics
- Bacteriophage lambda
- Blotting, Southern
- Chromosomes, Bacterial
- Cloning, Molecular
- Conjugation, Genetic
- DNA, Bacterial
- Escherichia coli
- Gene Expression
- Gene Rearrangement
- Genes, Bacterial
- Kinetics