A Rationally Designed Split Lettuce Aptamer Based on Large-Scale Mutational Analysis.

Abstract excerpt

Split-aptamer biosensors offer exceptionally low background by assembling only in the presence of a target analyte; however, their performance is frequently limited by the lack of robust design rules for selecting effective split sites. Existing approaches largely rely on heuristic, structure-based assumptions that are poorly validated and often yield suboptimal signal. Herein, we introduce a systematic,...

Topics

• Aptamers, Nucleotide
• Lactuca
• Biosensing Techniques
• SARS-CoV-2
• RNA, Viral
• Mutation
• Fluorescent Dyes