Insertion Pool Sequencing (iPool-Seq) for theHigh-Throughput Quantification of Ustilago maydis Mutant Fitness.

Abstract excerpt

Insertion pool sequencing (iPool-Seq) is a robust functional genomic technique that combines insertion mutagenesis with next-generation sequencing to comprehensively analyze the genome-wide gene function in maize infecting fungus Ustilago maydis. This high-throughput method tracks the abundance of individual mutants in pooled populations under defined selective conditions. It relies on the construction of...

Topics

• Ustilago
• High-Throughput Nucleotide Sequencing
• Zea mays
• Mutagenesis, Insertional
• Mutation
• Genome, Fungal