Article
Mu-seq: sequence-based mapping and identification of transposon induced mutations.
PloS one - 1 Jan 2013
McCarty Donald R, Latshaw Sue, Wu Shan, Suzuki Masaharu, Hunter Charles T, Avigne Wayne T, Koch Karen E
Abstract excerpt
Mutations tagged by transposon insertions can be readily mapped and identified in organisms with sequenced genomes. Collections of such mutants allow a systematic analysis of gene function, and can be sequence-indexed to build invaluable resources. Here we present Mu-seq (Mutant-seq), a high-throughput NextGen sequencing method for harnessing high-copy transposons. We illustrate the efficacy of Mu-seq by applying...
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