Article
TEAseq-based identification of 35,696 Dissociation insertional mutations facilitates functional genomic studies in maize.
Journal of genetics and genomics = Yi chuan xue bao - 20 Nov 2021
Lyu Mingjie, Liu Huafeng, Waititu Joram Kiriga, Sun Ying, Wang Huan, Fu Junjie, Chen Yanhui, Liu Jun, Ku Lixia, Cheng Xiliu
Abstract excerpt
In plants, transposable element (TE)-triggered mutants are important resources for functional genomic studies. However, conventional approaches for genome-wide identification of TE insertion sites are costly and laborious. This study developed a novel, rapid, and high-throughput TE insertion site identification workflow based on next-generation sequencing and named it Transposable Element Amplicon Sequencing...
Topics
- Chromosome Mapping
- DNA Transposable Elements
- Gene Library
- Genetic Association Studies
- Genome, Plant
- Genome-Wide Association Study
- Genomics
- High-Throughput Nucleotide Sequencing
- Mutagenesis, Insertional
- Phenotype
- Plants, Genetically Modified
- Polymorphism, Genetic