Article
Use of Illumina sequencing to identify transposon insertions underlying mutant phenotypes in high-copy Mutator lines of maize.
The Plant journal : for cell and molecular biology - 1 Jul 2010
Williams-Carrier Rosalind, Stiffler Nicholas, Belcher Susan, Kroeger Tiffany, Stern David B, Monde Rita-Ann, Coalter Robert, Barkan Alice
Abstract excerpt
High-copy transposons have been effectively exploited as mutagens in a variety of organisms. However, their utility for phenotype-driven forward genetics has been hampered by the difficulty of identifying the specific insertions responsible for phenotypes of interest. We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu)...
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