Article
SAturated Transposon Analysis in Yeast (SATAY) for Deep Functional Mapping of Yeast Genomes.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022
Michel Agnès H, Kornmann Benoît
Abstract excerpt
Genome-wide transposon mutagenesis followed by deep sequencing allows the genome-wide mapping of growth-affecting loci in a straightforward and time-efficient way.SAturated Transposon Analysis in Yeast (SATAY) takes advantage of a modified maize transposon that is highly mobilizable in S. cerevisiae. SATAY allows not only the genome-wide mapping of genes required for growth in select conditions (such as genetic...
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