Article
Expansion and improvement of ChinaMu by MuT-seq and chromosome-level assembly of the Mu-starter genome.
Journal of integrative plant biology - 1 Apr 2024
Liang Lei, Wang Yuancong, Han Yanbin, Chen Yicong, Li Mengfei, Wu Yibo, Ma Zeyang, Zhao Han, Song Rentao
Abstract excerpt
ChinaMu is the largest sequence-indexed Mutator (Mu) transposon insertional library in maize (Zea mays). In this study, we made significant improvements to the size and quality of the ChinaMu library. We developed a new Mu-tag isolation method Mu-Tn5-seq (MuT-seq). Compared to the previous method used by ChinaMu, MuT-seq recovered 1/3 more germinal insertions, while requiring only about 1/14 of the sequencing...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.