Random (UV) Mutagenesis, Unbiased Screening and Identification of DNA Repair Mutants by Complementation Cloning.

Abstract excerpt

As a highly radiation-resistant microbial BRCA2 organism, Ustilago maydis is an attractive genetic system to study the mechanisms underlying genome protection. Despite the completion of its genome sequence nearly two decades ago, the functions of many of its 6500+ genes remain unknown. This gap underscores the critical need to identify novel genes, some of which may play vital roles in safeguarding the genome....

Topics

• Mutagenesis
• DNA Repair
• Cloning, Molecular
• Ultraviolet Rays
• Ustilago
• Mutation
• Genetic Complementation Test