Article
Reaction Phenotyping of Low-Turnover Compounds in Long-Term Hepatocyte Cultures Through Persistent Selective Inhibition of Cytochromes P450.
Drug metabolism and disposition: the biological fate of chemicals - 1 Nov 2021
Smith Sheri, Lyman Michael, Ma Bennett, Tweedie Donald, Menzel Karsten
Abstract excerpt
Recognizing the challenges of determining the relative contribution of different drug metabolizing enzymes to the metabolism of slowly metabolized compounds, a cytochrome P450 reaction phenotyping (CRP) method using cocultured human hepatocytes (HEPATOPAC) has been established. In this study, the emphasis on the relative contribution of different cytochrome P450 (P450) isoforms was assessed by persistently...
Topics
- Algorithms
- Cells, Cultured
- Coculture Techniques
- Cytochrome P-450 Enzyme Inhibitors
- Cytochrome P-450 Enzyme System
- Drug Interactions
- Hepatocytes
- Humans
- Isoenzymes
- Microsomes, Liver
- Pharmaceutical Preparations
- Phenotype