Article
Two distinct Okazaki fragment failure modes dominate mutagenesis in the absence of flap endonuclease 1.
Nature communications - 17 Jul 2026
Lujan Scott A, Arana Mercedes E, Wilkins Hunter, Williams Jessica S, Garbacz Marta A, Bebenek Katarzyna, Kunkel Thomas A
Abstract excerpt
The yeast RAD27 gene encodes the Rad27/Fen1 nuclease, which contributes to genome stability during Okazaki fragment maturation (OFM), DNA mismatch repair (MMR), base excision repair, and other processes. Here, we analyze whole genome mutation accumulation in Saccharomyces cerevisiae lacking RAD27 and show that its loss elevates diverse mutation classes arising through multiple mechanistic pathways. Overall...
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