Article
Identification of rad27 mutations that confer differential defects in mutation avoidance, repeat tract instability, and flap cleavage.
Molecular and cellular biology - 1 Aug 2001
Xie Y, Liu Y, Argueso J L, Henricksen L A, Kao H I, Bambara R A, Alani E
Abstract excerpt
In eukaryotes, the nuclease activity of Rad27p (Fen1p) is thought to play a critical role in lagging-strand DNA replication by removing ribonucleotides present at the 5' ends of Okazaki fragments. Genetic analysis of Saccharomyces cerevisiae also has identified a role for Rad27p in mutation avoidance. rad27Delta mutants display both a repeat tract instability phenotype and a high rate of forward mutations to...
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