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Article

Engineering AaADH1 for enhanced catalytic efficiency and solubility via substrate tunnel modulation.

Enzyme and microbial technology - 1 Sept 2026

Wang Chenyu, Feng Xiao, Zong Lixia, Fan Shuai, Yan Maocai, Yang Zhaoyong

Abstract excerpt

Artemisinic alcohol dehydrogenase 1 (AaADH1) plays a pivotal role in the biosynthesis of artemisinic acid, a key precursor of the antimalarial drug artemisinin. However, its low catalytic activity and poor yield limit its industrial application. In this study, we employed a structure-guided protein engineering approach to improve the enzymatic properties of AaADH1. Substrate tunnel analysis identified key...

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Identifiers

PMID: 42418950
DOI: 10.1016/j.enzmictec.2026.110930

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

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