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Article

Enhancing the specific activity of 3α-hydroxysteroid dehydrogenase through cross-regional combinatorial mutagenesis.

International journal of biological macromolecules - 1 Dec 2024

Ma Siqi, Li Musen, Yan Shengheng, Wang Yi, Wang Binju, Luo Wei

Abstract excerpt

3α-Hydroxysteroid dehydrogenase (3α-HSD) from Comamonas testosteroni is widely used in clinical settings to measure serum total bile acid levels. However, its low enzymatic activity leads to high operational costs. In this study, we employed a combinatorial mutagenesis approach to systematically identify potential key mutation sites within the enzyme. The enzyme molecule was segmented into distinct regions, and a...

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PMID: 39486711
DOI: 10.1016/j.ijbiomac.2024.137014

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