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Article

Cross-region combinatorial mutagenesis reveals context-dependent enhancement of 3α-hydroxysteroid dehydrogenase catalytic efficiency.

International journal of biological macromolecules - 1 Sept 2026

Wang Junchao, Han Zhenlin, Wang Qiong, Luo Wei

Abstract excerpt

This study focused on enhancing the catalytic efficiency of 3α-hydroxysteroid dehydrogenase (3α-HSD) from Comamonas testosteroni toward androsterone. By employing enzyme engineering strategy combining semi-rational design with the protein language model Saprot, a high-performance mutant, H119A/R201L/R216L/N152F/K208A, was obtained. First, ethanol was used as a cosolvent to stabilize the reaction system, and the...

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Topics

3-Hydroxysteroid Dehydrogenases
Biocatalysis
Comamonas testosteroni
Mutagenesis
Mutation
Models, Molecular

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Kinetics

Protein Engineering

Catalytic Domain

Source

Identifiers

PMID: 42379440
DOI: 10.1016/j.ijbiomac.2026.152871

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