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Structure-Guided Engineering of KshA Yields a High-Performance 9α-Hydroxylase for Synthesis of 9α-OH-AD.

ACS synthetic biology - 20 Feb 2026

Dai Yanmei, Sun Dongchang, Zhang Qingyu, Tao Zijuan, Huang Changshun, Liu Bo, Ou Zhimin

Abstract excerpt

9α-hydroxy-4-androstene-3,17-dione (9α-OH-AD) is a crucial steroid pharmaceutical intermediate synthesized from androst-4-ene-3,17-dione (4-AD) via catalysis by 9α-hydroxylase (KSH), which comprises KshA and KshB subunits. KshB supplies electrons to activate the [2Fe-2S] center in KshA, enabling 4-AD hydroxylation. However, KSH's stability and activity limit industrial 9α-OH-AD production. This study identified...

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PMID: 41665121
DOI: 10.1021/acssynbio.5c00738

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