EMS mutation and SNP detection in intracellular Wolbachia genomes.

Abstract excerpt

Endosymbiotic bacteria such as Wolbachia pose significant challenges to genetic and molecular investigation due to their obligate intracellular lifestyle and complex growth requirements. Current understanding of their protein biology relies heavily on functional assignments inferred by homology, which may not reflect the specific roles endosymbiont proteins play within the host. This work addresses the need for...

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• Cell Line
• Drosophila melanogaster
• Ethyl Methanesulfonate
• Genome, Bacterial
• High-Throughput Nucleotide Sequencing