Article
Whole-Genome Sequence Accuracy Is Improved by Replication in a Population of Mutagenized Sorghum.
G3 (Bethesda, Md.) - 2 Mar 2018
Addo-Quaye Charles, Tuinstra Mitch, Carraro Nicola, Weil Clifford, Dilkes Brian P
Abstract excerpt
The accurate detection of induced mutations is critical for both forward and reverse genetics studies. Experimental chemical mutagenesis induces relatively few single base changes per individual. In a complex eukaryotic genome, false positive detection of mutations can occur at or above this mutagenesis rate. We demonstrate here, using a population of ethyl methanesulfonate (EMS)-treated Sorghum bicolor BTx623...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
