Discovery and engineering of a formate dehydrogenase with enhanced bicarbonate reduction activity.

Abstract excerpt

The enzymatic conversion of CO2 into value-added chemicals offers a promising strategy for carbon capture and utilization; however, the inherently low reductive activity of natural formate dehydrogenases (FDHs) limits their practical application. In this study, we employed an integrated approach combining deep learning, virtual saturation mutagenesis, and molecular dynamics simulations to discover and engineer...

Topics

• Formate Dehydrogenases
• Bicarbonates
• Oxidation-Reduction
• Protein Engineering
• Molecular Dynamics Simulation
• Molecular Docking Simulation