Article
Engineered formate dehydrogenase from Chaetomium thermophilum, a promising enzymatic solution for biotechnical CO2 fixation.
Biotechnology letters - 1 Nov 2020
Çakar Mehmet M, Ruupunen Jouni, Mangas-Sanchez Juan, Birmingham William R, Yildirim Deniz, Turunen Ossi, Turner Nicholas J, Valjakka Jarkko, Binay Barış
Abstract excerpt
OBJECTIVES: Formate dehydrogenases (FDHs) are NAD(P)H-dependent enzymes that catalyse the reversible oxidation of formate to CO2. The main goal was to use directed evolution to obtain variants of the FDH from Chaetomium thermophilum (CtFDH) with enhanced reduction activity in the conversion of CO2 into formic acid. RESULTS: Four libraries were constructed targeting five residues in the active site. We identified...
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