Directed evolution of an NAD-preferring shikimate dehydrogenase mutant for enhanced catalytic efficiency.

Abstract excerpt

Cofactor imbalance is a pervasive challenge in metabolic engineering, particularly when microbial hosts with native NADH-dominated redox pools express heterologous pathways requiring NADPH. A prominent example is the shikimate pathway, where NADPH-dependent shikimate dehydrogenase (SDH) constitutes a bottleneck. Although rational design has attempted to shift SDH specificity toward NADH, the resulting mutants...

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• Alcohol Oxidoreductases
• Biocatalysis
• Directed Molecular Evolution
• Escherichia coli
• Kinetics