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Article

SARS-CoV-2 Evolution and Its Implications for RT-PCR Diagnostic Performance.

Journal of medical virology - 1 Jun 2026

Gupta Shubhangi, Chaudhary Abhishek, Bhatnagar Sonika

Abstract excerpt

Mutations in SARS-CoV-2 primer/probe targets can compromise RT-PCR sensitivity, leading to false negatives and undetected viral transmission. This study evaluated mutational landscapes of 20 primer/probe sets targeting E, N, Orf1b-nsp14, RdRp, and S genes using 1,35,852 high-quality genomes across ten countries, five major variants, and three time periods (pre-vaccination, post-vaccination, and recent). Mutations...

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Share to Coronavirus Nucleocapsid Proteins
Share to COVID-19
Share to Genome, Viral

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutation Rate

Share to RNA, Viral

Share to SARS-CoV-2

Share to Sensitivity and Specificity

Share to Spike Glycoprotein, Coronavirus

Source

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Identifiers

PMID: 42312714
DOI: 10.1002/jmv.71023
PMCID: PMC13277296

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

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