Article

Evaluation of WHO listed COVID-19 qPCR primers and probe in silico with 375 SERS-CoV-2 full genome sequences

2020-04-28

Abstract excerpt

Quantitative reverse-transcription PCR (qRT-PCR) assays remains the gold standard for detection of the SARS-CoV-2 virus because of its sensitivity and specificity. However, successful design of qRT-PCR assays requires accurate viral genome sequences. With mutations accumulating as the virus is transmitted globally, we sought to compare current assays recommended by the World Health Organization with available SARS...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: d8dabb27-fae2-5048-85a9-26a7cd128d1b
DOI: 10.1101/2020.04.22.20075697

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Evaluation of WHO listed COVID-19 qPCR primers and probe in silico with 375 SERS-CoV-2 full genome sequencesDOI 10.1101/2020.04.22.20075697Read

Validation of RT-qPCR primers and probes for new and old variants of SARS-CoV-2 in a world scaleSemantic proximity Read

Analysis of the potential impact of genomic variants in global SARS-CoV-2 genomes on molecular diagnostic assaysSemantic proximity Read

A one-step, one-tube real-time RT-PCR based assay with an automated analysis for detection of SARS-CoV-2Semantic proximity Read

Comparison of three molecular diagnostic assays for SARS‐CoV‐2 detection: Evaluation of analytical sensitivity and clinical performanceSemantic proximity Read

True or false: what are the factors that influence COVID-19 diagnosis by RT-qPCR?Semantic proximity Read

How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection? Semantic proximity Read

Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants.Semantic proximity Read

Highly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratoriesSemantic proximity Read

Presence of mismatches between diagnostic PCR assays and coronavirus SARS-CoV-2 genomeSemantic proximity Read

Comparison and Sensitivity Evaluation of Three Different Commercial Real-Time Quantitative PCR Kits for SARS-CoV-2 DetectionSemantic proximity Read

Advancements in detection of SARS-CoV-2 infection for confronting COVID-19 pandemics.Semantic proximity Read

Diagnostic usefulness of subgenomic RNA detection of viable SARS-CoV-2 in patients with COVID-19Semantic proximity Read