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Article

The Impact of Accumulated Mutations in SARS-CoV-2 Variants on the qPCR Detection Efficiency.

Frontiers in cellular and infection microbiology - 1 Jan 2022

Cao Liu, Xu Tiefeng, Liu Xue, Ji Yanxi, Huang Siyao, Peng Hong, Li Chunmei, Guo Deyin

Abstract excerpt

SARS-CoV-2 is evolving with mutations throughout the genome all the time and a number of major variants emerged, including several variants of concern (VOC), such as Delta and Omicron variants. In this study, we demonstrated that mutations in the regions corresponding to the sequences of the probes and 3'-end of primers have a significant impact on qPCR detection efficiency. We also found that the G28916T...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 35155280
DOI: 10.3389/fcimb.2022.823306
PMCID: PMC8834649

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