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Article

Evolving strategy for an evolving virus: Development of real-time PCR assays for detecting all SARS-CoV-2 variants of concern.

Journal of virological methods - 1 Sept 2022

Pabbaraju Kanti, Zelyas Nathan, Wong Anita, Croxen Matthew A, Lynch Tarah, Buss Emily, Murphy Stephanie, Shokoples Sandy, Kanji Jamil, Tipples Graham

Abstract excerpt

In order to detect the SARS-CoV-2 variants of concern (VOCs), five real-time reverse transcriptase PCR (rRT-PCR) assays were designed to target the critical discriminatory mutations responsible for the following amino acid changes in the spike protein: two Δ69-70 + N501Y + E gene triplexes (one optimized for Alpha [B.1.1.7] and one optimized for Omicron [B.1.1.529]), a K417N + 242-244 wild-type duplex, a K417T...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to RNA, Viral

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Source

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Identifiers

PMID: 35644262
DOI: 10.1016/j.jviromet.2022.114553
PMCID: PMC9134755

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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