Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Assay-dependent discordance in detecting a rare HLA-DPB1 allele: Long-range PCR vs. hybrid capture NGS.

Human immunology - 1 Aug 2026

Rozar Katie, Cano Pedro, Verma Deepak, Wendel Jennifer, Iozzino Crystal

Abstract excerpt

Next-generation sequencing (NGS) has become fundamental to high-resolution HLA typing, but methodological differences between long-range PCR (LR PCR) and hybrid capture (HC) approaches can lead to discrepant results. This study examined cases where an allele identified as DPB1*13:01 by LR PCR was assigned DPB1*107:01 by HC. This discordance was attributed to both primer-site mutations and incomplete coverage of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to High-Throughput Nucleotide Sequencing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Histocompatibility Testing

Share to HLA-DP beta-Chains

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 42214264
DOI: 10.1016/j.humimm.2026.111769

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Assay-dependent discordance in detecting a rare HLA-DPB1 allele: Long-range PCR vs. hybrid capture NGS.PMID 42214264Read ↗

Comparison of sequence‐specific oligonucleotide probe vs next generation sequencing for HLA‐A, B, C, DRB1, DRB3/B4/B5, DQA1, DQB1, DPA1, and DPB1 typing: Toward single‐pass high‐resolution HLA typing in support of solid organ and hematopoietic cell transplant programsSemantic proximity Read ↗

Targeted Next-Generation Sequencing for Human Leukocyte Antigen Typing in a Clinical Laboratory: Metrics of Relevance and Considerations for Its Successful Implementation.Semantic proximity Read ↗

Application of High-Throughput Next-Generation Sequencing for HLA Typing on Buccal Extracted DNA: Results from over 10,000 Donor Recruitment Samples.Semantic proximity Read ↗

Reduced PCR-generated errors from a hybrid capture-based NGS assay for HLA typing.Semantic proximity Read ↗

High-throughput next-generation sequencing to genotype six classical HLA loci from 96 donors in a single MiSeq run.Semantic proximity Read ↗

Determining performance characteristics of an NGS-based HLA typing method for clinical applications.Semantic proximity Read ↗

Concurrent typing of over 4000 samples by long-range PCR amplicon-based NGS and rSSO revealed the need to verify NGS typing for HLA allelic dropouts.Semantic proximity Read ↗

Population-agnostic real-time molecular mismatch estimation using rSSO-defined HLA allele strings: minimal impact of allelic discordance with NGS.Semantic proximity Read ↗

Validation and application of new NGS-based HLA genotyping to clinical diagnostic practice.Semantic proximity Read ↗

Patterns of non-ARD variation in more than 300 full-length HLA-DPB1 alleles.Semantic proximity Read ↗

Buccal swab genomic DNA fragmentation predicts likelihood of successful HLA genotyping by next-generation sequencing.Semantic proximity Read ↗

Next generation sequencing to determine HLA class II genotypes in a cohort of hematopoietic cell transplant patients and donors.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.