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Article

Engineering of glycoside hydrolase family 7 cellobiohydrolases directed by natural diversity screening.

The Journal of biological chemistry - 1 Mar 2024

Brunecky Roman, Knott Brandon C, Subramanian Venkataramanan, Linger Jeffrey G, Beckham Gregg T, Amore Antonella, Taylor Larry E, Vander Wall Todd A, Lunin Vladimir V, Zheng Fei, Garrido Mercedes, Schuster Logan, Fulk Emily M, Farmer Samuel, Himmel Michael E, Decker Stephen R

Abstract excerpt

Protein engineering and screening of processive fungal cellobiohydrolases (CBHs) remain challenging due to limited expression hosts, synergy-dependency, and recalcitrant substrates. In particular, glycoside hydrolase family 7 (GH7) CBHs are critically important for the bioeconomy and typically difficult to engineer. Here, we target the discovery of highly active natural GH7 CBHs and engineering of variants with...

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Identifiers

PMID: 38354778
DOI: 10.1016/j.jbc.2024.105749
PMCID: PMC10943489

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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