Free energy-guided Q302P mutation in GH1 β-glucosidase from Aspergillus fumigatus improves enzyme function via catalytic tunnel perturbation.

Abstract excerpt

Beta-glucosidase (EC 3.2.1.21) is crucial to biorefinery industries because it is the bottleneck enzyme in the enzymatic conversion of cellobiose to glucose, a key component of the enzyme complex that converts lignocellulosic biomass into glucose. The enzyme is inhibited by its own product, glucose, which accumulates and blocks the catalytic tunnel or the product exit region. This is the main drawback of this...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Aspergillus fumigatus
• Share to beta-Glucosidase