Enhancing PgUGT activity and thermostability via mutation site screening aided by molecular docking structure and computer-aided design for efficient industrial synthesis of rebaudioside D.

Abstract excerpt

Rebaudioside D (Reb D) is a natural high-intensity, zero-calorie sweetener with a superior taste profile compared to stevioside and rebaudioside A (Reb A), creating high consumer demand. In this study, the glycosyltransferase PgUGTM0 was engineered via rational design and computational strategies to enhance the biocatalytic production of Reb D. A combinatorial mutant, PgUGTM2...

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