Article
Enhancing the Catalytic Activity of Thermococcus kodakarensis RuBisCo via Computer-Aided Rational Design.
Journal of agricultural and food chemistry - 6 May 2026
Li Sainan, Zhou Jia, Song Xiangfei, Chen Jingfei, Wang Yefei, Yao Lishan
Abstract excerpt
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) is a prime target for enhancing the photosynthetic efficiency. Here, we employed bmDCA, a machine-learning implementation, to navigate the sequence space of Thermococcus kodakarensis RuBisCo (Tk-RuBisCo). After only two design-test cycles involving ∼30 variants, we isolated the double mutant SP9-Y168L-A361P, which exhibits a 1.5-fold increase in activity...
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