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Article

Directed Evolution of an Improved Rubisco; In Vitro Analyses to Decipher Fact from Fiction.

International journal of molecular sciences - 10 Oct 2019

Zhou Yu, Whitney Spencer

Abstract excerpt

Inaccuracies in biochemically characterizing the amount and CO2-fixing properties of the photosynthetic enzyme Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase continue to hamper an accurate evaluation of Rubisco mutants selected by directed evolution. Here, we outline an analytical pipeline for accurately quantifying Rubisco content and kinetics that averts the misinterpretation of directed evolution...

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Source

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Identifiers

PMID: 31658746
DOI: 10.3390/ijms20205019
PMCID: PMC6834295

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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