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Article

Computational-guided semi-rational design to reprogram phospholipase D into industrially robust enzyme.

Bioresource technology - 1 Jul 2026

Kong Xiao-Li, Zhang Peng, Qin Zheng-Qiang, Su Chang, Li Heng, Lu Zhen-Ming, Gong Jin-Song, Xu Zheng-Hong, Shi Jin-Song

Abstract excerpt

Phospholipase D (PLD) catalyzes the synthesis of rare phospholipid derivatives from phospholipids, and these derivatives possess significant application value and market potential. However, most reported PLDs show poor thermal stability, with melting temperatures below 60 °C, limiting their industrial application. This study employed a computer-aided design strategy integrating ancestral sequence reconstruction...

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PMID: 41916458
DOI: 10.1016/j.biortech.2026.134528

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