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Article

Increasing the flexibility of the substrate binding pocket of Streptomyces phospholipase D can enhance its catalytic efficiency in soybean phosphatidylcholine.

International journal of biological macromolecules - 1 Nov 2024

Hu Rongkang, Cao Jiale, Rong Chenghao, Wu Siyi, Wu Linxiu

Abstract excerpt

The catalytic efficiency of Streptomyces klenkii phospholipase D (SkPLD) in soybean phosphatidylcholine (soy-PC) processing is constrained by its acyl chain specificity. To address this limitation, we engineered the substrate-binding pocket of SkPLD to increase its flexibility. The mutant P343A/Y383L exhibited a 7.14-fold increase in catalytic efficiency toward soy-PC compared to the wild type. This enhancement...

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PMID: 39306159
DOI: 10.1016/j.ijbiomac.2024.135824

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